Assembly of glucose oxidase and different polyelectrolytes by means of electrostatic layer by-layer adsorption on a thiolated gold surface

MARCELA C. RODRIGUEZ; GUSTAVO A. RIVAS

ELECTROANALYSIS

Wiley Interscience

Lugar: Weinheim; Año: 2004 vol. 16 p. 1717 - 1717

1040-0397

p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt; TEXT-ALIGN: justify; mso-layout-grid-align: none">A rational strategy for the construction of a bioelectrocatalytic architecture by means of alternate electrostatic adsorption is described. Multilayer films containing glucose oxidase (GOx) and different polyelectrolytes were assembled onto a thiolated-gold surface and the resulting bioelectrode was used for glucose biosensing. The supramolecular multistructure was prepared by assembling polyethylenimine and Nafion (as anti-interference barrier), followed by the adsorption of polyethylenimine and DNA (as stabilizing film) and finally by the alternate deposition of polyethylenimine and glucose oxidase (as a biocatalytic layer). The influence of the deposition time and concentration of polyelectrolytes, organization and number of layers on the sensitivity and selectivity of the bioelectrode is discussed. T